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Image Search Results
Journal: Antiviral research
Article Title: A cell-based high throughput screening assay for the discovery of cGAS-STING pathway agonists
doi: 10.1016/j.antiviral.2017.10.001
Figure Lengend Snippet: THF cells were left untreated or treated with indicated concentrations of DSDP or control compound G10 for 8 h. Cells were then infected with YFV, DENV, or ZIKV at MOI of 0.1 for 1 h followed by culture for additional 48 h. Total cellular RNAs were extracted to detect viral genomic RNA by qRT-PCR using β-actin as internal control (A, C and E). Values represented percentage to mock treated controls (mean ±standard derivations, n=3). Culture media were harvested and the virus titers (B, D and F) were determined by plaque assay and presented as number of plaque forming units per milliliter medium (PFU/ml) (mean ±standard derivations, n=3). UD, under detection limit. * indicates p<0.05 compared to mock treated control.
Article Snippet: Sendai virus (SenV, strain 52) and human serum derived PRVABC59 strain of
Techniques: Control, Infection, Quantitative RT-PCR, Virus, Plaque Assay